Needle stick injury is a percutaneous piercing wound typically caused by needle point and other sharp instruments or objects. The injuries may expose the individual to blood-borne pathogens like Hepatitis B, Hepatitis C and Human Immunodeficiency Virus (HIV). Nursing students, due to their limited clinical experience and developing skills, are especially vulnerable to needle stick injuries. Therefore, the present study aims to assess the effectiveness of educational package on knowledge regarding needle stick injury among GNM students. A quantitative research approach and pre-experimental one group pre-test post-test design was adopted for the study. The study was conducted among 40 GNM students selected through a non-probability, convenience sampling technique at a selected college of nursing in Lucknow. Data were collected using a self-structured questionnaire and educational package was delivered. The results reveal that in the pre-test, the majority of students (19, 47.5%) had average knowledge, 16 (40%) had good knowledge, and 5 (12.5%) had poor knowledge regarding needle stick injuries. After the intervention, in post-test, 27(65.5%) had good knowledge, 11(27.5%) had excellent knowledge and 2(5%) had average knowledge regarding needle stick injury. The pre-intervention mean knowledge level was 9.38±3.36, and after the intervention, it was found to be 14.16±2.62, indicating a change of 4.78 in knowledge level, which was statistically significant. The paired “t” value of 9.04 reveals effectiveness of educational package on knowledge regarding needle stick injury at 0.05 level of significance. Thus, the study concluded that educational package was effective in increasing the knowledge regarding needle stick injury among GNM students.