author(s)
Amaravathi D, Jaikumar J and Rajalakshmi S

abstract
Cryopreservation of biological tissues, cells, or organs at ultra-low temperatures is increasingly vital in paediatric care for children at risk of fertility or immune impairment due to medical treatments. Advances in verification, cryoprotectants, and bio banking have significantly enhanced the viability and safety of specimens, including hematopoietic stem cells, ovarian and testicular tissue, mature gametes, and cord blood. Nurses play a central role in this process, providing patient and family education, facilitating informed consent and assent, coordinating pre, intra, and post-procedure care, ensuring specimen handling and documentation, and offering long-term follow-up and psychosocial support. Ethical and legal considerations, including autonomy, confidentiality, tissue ownership, and regulatory compliance, are integral to the practice. Case scenarios demonstrate how nursing interventions support families through fertility preservation. Overall, nurses ensure holistic, family-centered care that safeguards patient rights, enhances outcomes, and preserves future health and reproductive options.