Background: Children are the important asset of the nation. Parents play a key role in toilet training they need to provide their children with direction, motivation and reinforcement. Parents have an important role in the toilet training of the children.

Objectives: To assess knowledge regarding toilet training among mothers of under five before and after administration of structured teaching programme.

Methods: A Quantitative research approach with pre test post design was used. 60 mothers were selected as a sample in the study using consecutive sampling technique. Structured questionaries was used. The structured teaching programme regarding toilet training was prepared by researcher which. The pre-test was conducted followed by Structured teaching programme was administered and the post assessment-I was assessed immediately after the completion of Structured teaching programme.

Results: Findings of the study indicate that structured teaching programme was effective in enhancing the knowledge as the findings indicate that the mean of the post-test knowledge score (19.16) was higher by pre-test knowledge score which was (7.35).

Conclusion: Structured teaching programme was effective in enhancing the knowledge of mothers regarding toilet training.