Cervical cancer is second most common cancer in the world and leading cause of death from cancer among women in developing countries. The objectives of the study were to compare knowledge regarding cervical cancer among women of reproductive age in selected rural and urban areas of Maharashtra state. A comparative study was conducted using descriptive, cross sectional survey among conveniently sampled 120 women; 60 from rural and 60 from urban areas. A structured interview schedule with 25 questions validated by experts were used for data collection. The study results revealed that majority of subjects had poor knowledge (68.3% rural and 55% urban). So there is a need to raise public awareness regarding prevention of cervical cancer.