Intrahospital transport refers to the transferring of patients within and around (intra) the hospital. The research study was conducted to develop and validate intrahospital transport protocol of hospitalized patients for staff nurses in a selected cancer hospital, Kolkata. It was a methodological study conducted in two phase: In the first phase, survey design was followed to collect the data. In the second phase, based on the study findings Intrahospital transport protocol was developed and validated by the experts. Sample size for the study consisted of 100 situation of intrahospital transportation in presence of the staff nurses of Tata medical center, Kolkata. The sampling technique was non- probability purposive sampling. Tools were validated by 11 experts. “Structured interview” used to assess the self-reported practice of staff nurses and “Observation checklist” was used containing forty-four items. After analysis of the results “Intrahospital transport protocol” was developed in the form of SOP (Standard operating procedures). Protocol was divided in three sections: Procedure before transport, during transport and post transport. It contains 109 items out of which 63 items to be followed before transportation, 9 items during transportation and 16 items after transportation. Protocol was validated by five experts from nursing administrator field. Out of 109 items, only 3 items had 80% agreement and 106 items had 100% agreement. Overall Agreement of tool was calculated as 99.44% for all the items.