Objectives: A cross-sectional study was conducted to evaluate the factors influencing food handlers' understanding of food hygiene in selected urban areas of Karnataka.

Methodology: The research employed a descriptive approach, focusing on 10 urban areas in Karnataka. Using a multi-stage sampling technique, 100 food handlers were selected. Data collection was performed using a structured knowledge questionnaire comprising two sections.

Findings: The study revealed an overall knowledge mean score of 22.64 (SD±5.580), indicating inadequate food hygiene knowledge among food handlers. Of the 100 participants, 70(70%) demonstrated inadequate knowledge, 29(29%) showed moderately adequate knowledge, and only 1(1%) possessed adequate knowledge. A significant association was found between knowledge and training (p = 0.005).

Conclusion: The study's results highlighted that a majority (70%) of food handlers had insufficient knowledge of food hygiene. This finding highlights the critical importance of fostering awareness about food hygiene practices. By implementing comprehensive educational programs and outreach initiatives, empowering the food handles with essential knowledge regarding safe food handling, proper cooking temperatures, and effective sanitation methods. Such proactive measures are vital in mitigating the risks associated with food poisoning and food-borne illnesses, ultimately leading to healthier communities and improved public health outcomes.