Aim: The aim of the study was to assess the level of nurses’ knowledge regarding infection control at National Institute of Diseases of the Chest and Hospital.

Methods: A descriptive type of cross-sectional study was carried out from July, 2023 to June, 2024 among 50 nurses who were selected conveniently from 200 nurses. A self-administering structured questionnaire was used for data collection and descriptive statistics (frequency, percentage and mean) was used for data analysis.

Results: The mean age of the respondents was 36.02 years. Majority (92%) of the respondents were female and 64% were Muslim and only 10% were unmarried. More than half (54%) of the respondents did not have any special training on infection control. The overall knowledge level of the nurses showed that about 60% nurses had average level of knowledge on infection control.

Conclusion and Recommendation: Nurses’ level of knowledge regarding infection control is not satisfactory. So, organizing regular training on ICP, monitoring, development and implementation of guideline to improve knowledge of nurses for infection control in the hospital.