Methods: Research design; a pre experimental one group pre-test post-test design was used to carry out the study. Setting: The study was conducted in Shri. J.G.C.H.S. College of Nursing, Ghataprabha, Belagavi. Students: simple random sampling technique of 2^nd and 3^rd year B. Sc. Nursing students (50).

Tool: Part-I structured questionnaire on demographic data. Part-II structured knowledge questionnaire to assess the level of knowledge regarding HBOT.

Results: The pre-test findings revealed that majority (60%) of the students were having average knowledge and 40% of the students were having the poor knowledge regarding HBOT, with the mean score of 14.50 ± 3.564. The findings from post-test represents that majority (68%) were having good knowledge and 32% of the respondents were having the average knowledge regarding HBOT with the mean score of 34.40 ± 2.330. The significant association was found between Gender and post-test knowledge scores (x² = 3.687 df=1 p< 0.05).

Conclusion: The investigator concluded that the video-assisted teaching programme can improve the knowledge regarding Hyper baric oxygen therapy among B.Sc. Nursing students.